- Data Analysis
- Other Protocols
Western Blot Data Analysis
In order for the results of a western blot to be useful, to major things must be true:
- The film must be correctly underexposed.
- You must be able to make relative comparisons between the bands.
You’ll notice that the second condition necessarily depends on the first comparison. So we’ve included a brief discussion how to correctly expose your western blots to film here:
Relative comparisons between western blot samples are critical, and we can use “densitometry” to help us make our comparisons relatively quantitative.