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A definitive guide to western blotting technique.
Thanks for visiting How to Western Blot. We have created an instructional, web-based resource for the easy standardization of western blots. Perfecting the western blot technique can be a time-consuming and frustrating process, but it does not need to be!
The western blot is one of the most commonly used techniques in molecular biology, and, as such, has been standardized a countless number of times. We believe that the knowledge gained from years of western blotting should be shared in an easy to understand and accessible manner, so that lab hours and government funding is not wasted on standardizing techniques that have already been standardized.
We also have a full protocol for standard western blots in our protocol section.
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Finally, we would love to see your success at the bench! We would love to see the differences between the standard protocol, and the bis-tris immunoblotting protocol. Please email us or use the contact form to inform us how the protocols are working for you.
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